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The dark matter halo of the Milky Way is predicted to contain a very largenumber of smaller subhalos. As a result of the dark matter annihilations takingplace within such objects, the most nearby and massive subhalos could appear aspoint-like or spatially extended gamma-ray sources, without observablecounterparts at other wavelengths. In this paper, we use the results of theAquarius simulation to predict the distribution of nearby subhalos, and comparethis to the characteristics of the unidentified gamma-ray sources observed bythe Fermi Gamma-Ray Space Telescope. Focusing on the brightest high latitudesources, we use this comparison to derive limits on the dark matterannihilation cross section. For dark matter particles lighter than ~200 GeV,the resulting limits are the strongest obtained to date, being modestly morestringent than those derived from observations of dwarf galaxies or theGalactic Center. We also derive independent limits based on the lack ofunidentified gamma-ray sources with discernible spatial extension, but theselimits are a factor of ~2-10 weaker than those based on point-like subhalos.Lastly, we note that four of the ten brightest high-latitude sources exhibit asimilar spectral shape, consistent with 30-60 GeV dark matter particlesannihilating to b quarks with an annihilation cross section on the order ofsigma v ~ (5-10) x 10^-27 cm^3/s, or 8-10 GeV dark matter particlesannihilating to taus with sigma v ~ (2.0-2.5) x 10^-27 cm^3/s.

Stringent Constraints On The Dark Matter Annihilation Cross Section From Subhalo Searches With The Fermi Gamma-Ray Space Telescope