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In a recent paper [arXiv:0904.2904] using a conjecture it is shown how onecan calculate the effect of a weak stationary gravitational field on vacuumenergy in the context of Casimir effect in an external gravitational fieldtreated in 1+3 formulation of spacetime decomposition.. In this article,employing quntum field theory in curved spacetime, we explicitly calculate theeffect of a weak static gravitational field on virtual massless scalarparticles in a Casimir apparatus. It is shown that, as expected from theproposed conjecture, both the frequency and renormalized energy of the virtualscalar field are affected by the gravitational field through its index ofrefraction. This could be taken as a strong evidence in favour of the proposedconjecture. Generalizations to weak {\it stationary} spacetimes and virtualphotons are also discussed.

Casimir effect in a weak gravitational field and the spacetime index of refraction