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We present the results of our QCD analysis for nonsinglet unpolarized quarkdistributions and structure function $F_2(x,Q^2)$. New parameterizations arederived for the nonsinglet quark distributions for the kinematic wide range of$x$ and $Q^2$. The analysis is based on the Jacobi polynomials expansion of thestructure function. The higher twist contributions of proton and deuteronstructure function are obtained in the large $x$ region. Our calculations fornonsinglet unpolarized quark distribution functions based on the Jacobipolynomials method are in good agreement with the other theoretical models. Thevalues of $\Lambda_{QCD}$ and $\alpha_s(M_z^2)$ are determined.

NNLO QCD contributions to the flavor non-singlet sector of ${\bf {F_2(x,Q^2)}}$