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Based on the isospin-dependent Boltzmann-Uehling-Uhlenbeck(IBUU04) transportmodel, we explored effects of in-medium $NN$ elastic scattering cross sectionand nuclear symmetry energy on the sub-threshold pion production in$^{132}Sn+^{124}Sn$ reaction. We find that with decrease of incident beamenergy, effects of in-medium $NN$ elastic scattering cross section on the$\pi^{-}/\pi^{+}$ ratio are larger than that of the symmetry energy althoughthe latter may be also larger. While keeping the effect of symmetry energy, thedouble ratio of $\pi^{-}/\pi^{+}$ from neutron-rich and neutron-poor reactionsystems (with the same mass number of system) $^{132}Sn+^{124}Sn$ and$^{128}Pm+^{128}Pm$ almost fully cancels out the effects of in-medium $NN$elastic scattering cross section.

Effects of nuclear symmetry energy and in-medium NN cross section in heavy-ion collisions at beam energies below the pion production threshold