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Due to its superior coherent and optical properties at room temperature, thenitrogen-vacancy (N-V ) center in diamond has become a promising quantum probefor nanoscale quantum sensing. However, the application of N-V containingnanodiamonds to quantum sensing suffers from their relatively poor spincoherence times. Here we demonstrate energy efficient protection of N-V spincoherence in nanodiamonds using concatenated continuous dynamical decoupling,which exhibits excellent performance with less stringent microwave powerrequirement. When applied to nanodiamonds in living cells we are able to extendthe spin coherence time by an order of magnitude to the $T_1$-limit of up to$30\mu$s. Further analysis demonstrates concomitant improvements of sensingperformance which shows that our results provide an important step towards invivo quantum sensing using N-V centers in nanodiamond.

Protecting quantum spin coherence of nanodiamonds in living cells