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We have realized a one dimensional optical lattice for individual atoms witha lifetime >300 s, which is 5 times longer than previously reported. In orderto achieve this long lifetime, it is necessary to laser cool the at-oms brieflyevery 20 s to overcome heating due to technical fluctuations in the trappingpotential. Without cooling, we observe negligible atom loss within the first 20s followed by an exponential decay with a 62 s time constant. We obtainquantitative agreement with the measured fluctuations of the trapping potentialand the corresponding theoretical heating rates.

Achieving very long lifetimes in optical lattices with pulsed cooling