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Neospora  caninum  primarily infects cattle, causing abortions, with an estimated impact of a billion dollars on the worldwide economy annually. However, the study of its biology has been unheeded by the established paradigm that it is virtually identical to its close relative, the widely studied human pathogen  Toxoplasma gondii . By revisiting the genome sequence, assembly, and annotation using third-generation sequencing technologies, here we show that the  N. caninum  genome was originally incorrectly assembled under the presumption of synteny with  T. gondii . We show that major chromosomal rearrangements have occurred between these species. Importantly, we show that chromosomes originally named Chr VIIb and VIII are indeed fused, reducing the karyotype of both  N. caninum  and  T. gondii  to 13 chromosomes. We reannotate the  N. caninum  genome, revealing more than 500 new genes. We sequence and annotate the nonphotosynthetic plastid and mitochondrial genomes and show that although apicoplast genomes are virtually identical, high levels of gene fragmentation and reshuffling exist between species and strains. Our results correct assembly artifacts that are currently widely distributed in the genome database of  N. caninum  and  T. gondii  and, more importantly, highlight the mitochondria as a previously oversighted source of variability and pave the way for a change in the paradigm of synteny, encouraging rethinking the genome as basis of the comparative unique biology of these pathogens.

genome research

Reevaluation of the <i>Toxoplasma gondii</i> and <i>Neospora caninum</i> genomes reveals misassembly, karyotype differences, and chromosomal rearrangements

Reevaluation of the Toxoplasma gondii and Neospora caninum genomes reveals misassembly, karyotype differences, and chromosomal rearrangements