English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Telomerase replenishes telomere tracts by reiteratively copying its RNA template, TER. Unlike other model organisms,  Arabidopsis thaliana  harbors two divergent  TER  genes. However, only  TER1  is required for telomere maintenance. Here we examine the function of  TER2 . We show that TER2 is spliced and its 3′ end is truncated in vivo to generate a third TER isoform, TER2 S . TERT preferentially associates with TER2 &gt; TER1 &gt; TER2 S . Moreover, TER2 and TER2 S  assemble with Ku and POT1b (protection of telomeres), forming RNP (ribonucleoprotein) complexes distinct from TER1 RNP. Plants null for  TER2  display increased telomerase enzyme activity, while  TER2  overexpression inhibits telomere synthesis from TER1 and leads to telomere shortening. These findings argue that TER2 negatively regulates telomerase by sequestering TERT in a nonproductive RNP complex. Introduction of DNA double-strand breaks by zeocin leads to an immediate and specific spike in TER2 and a concomitant decrease in telomerase enzyme activity. This response is not triggered by replication stress or telomere dysfunction and is abrogated in  ter2  mutants. We conclude that  Arabidopsis  telomerase is modulated by TER2, a novel DNA damage-induced noncoding RNA that works in concert with the canonical TER to promote genome integrity.

An alternative telomerase RNA in Arabidopsis modulates enzyme activity in response to DNA damage