Open AccessA novel recombinant of Hepatitis B virus genotypes G and C isolated from a Thai patient with hepatocellular carcinoma
Author(s) -
Kamol Suwannakarn,
Pisit Tangkijvanich,
Apiradee Theamboonlers,
Kenji Abe,
Yong Poovorawan
Publication year - 2005
Publication title -
journal of general virology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.55
H-Index - 167
eISSN - 1465-2099
pISSN - 0022-1317
DOI - 10.1099/vir.0.81241-0
Subject(s) - genotype , hepatocellular carcinoma , virology , biology , hepatitis b virus , phylogenetic tree , recombinant dna , orthohepadnavirus , gene , hepatitis c virus , genome , hepadnaviridae , virus , genetics
Genomic recombination between different genotypes of Hepatitis B virus (HBV) resulting in hybrid strains has been increasingly documented. In this study, a novel recombinant of HBV genotypes G and C isolated from a Thai patient with hepatocellular carcinoma is reported. Based on phylogenetic analyses of the S, P and X genes and the entire genome, the HBV isolate clustered on a branch within genotype G, but clustered with genotype C on analysis of the C gene. Using the program simplot and bootscanning analysis, the recombination breakpoints were located at nt 1860 and 2460 of the precore/core region. The hallmarks of the original genotype G, including a 36 bp insertion in the core region and dual stop codons in the precore region, were not identified in this isolate. These data should encourage further investigations on the epidemiological and virological characteristics of HBV genotype G involved in recombination with other genotypes.
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