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Mycobacterium tuberculosis FtsZ (MtbFtsZ) exhibits unusually slow polymerization kinetics in vitro (White et al., 2000). Recently, Anand et al. (2004) hypothesized that removal of the non-conserved C terminus would bestow rapid polymerization to MtbFtsZ, comparable to the much faster-polymerizing Escherichia coli FtsZ (EcFtsZ) (Mukherjee & Lutkenhaus, 1999). Indeed, MtbFtsZ(DC169), in which the last 169 residues were deleted (D211–379; Fig. 1), was found to polymerize much faster than wild-type MtbFtsZ (Anand et al., 2004). It also formed polymers that were 1?5-times thicker and that exhibited an altered morphology, compared with wild-type MtbFtsZ protofilaments (White et al., 2000). We show here, however, that the three-dimensional structure of MtbFtsZ(DC169) is so perturbed – the truncation exposes a large hydrophobic patch that is normally buried (Fig. 2) – that polymerization of MtbFtsZ(DC169) is unlikely to be physiologically relevant. The cause(s) of the slow polymerization of wild-type MtbFtsZ must therefore be sought elsewhere.

Polymerization of C-terminally truncated Mycobacterium tuberculosis FtsZ is unlikely to be physiologically relevant