Open AccessEvaluation of a commercial real-time PCR for the detection of extended spectrum β-lactamase genes
Author(s) -
Ina Willemsen,
Linda Hille,
Angela C.I.M. Vrolijk,
Anneke Bergmans,
Jan Kluytmans
Publication year - 2014
Publication title -
journal of medical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.91
H-Index - 117
eISSN - 1473-5644
pISSN - 0022-2615
DOI - 10.1099/jmm.0.070110-0
Subject(s) - enterobacteriaceae , gold standard (test) , real time polymerase chain reaction , polymerase chain reaction , biology , gene , microbiology and biotechnology , genetics , escherichia coli , statistics , mathematics
We investigated the performance of a real-time PCR for the detection of extended spectrum β-lactamase genes in Enterobacteriaceae (Check-MDR ESBL PCR). Results from micro-arrays were considered as the gold standard. An analysis on 489 isolates resulted in a sensitivity of 98.9 % and a specificity of 100 % for the PCR.
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