Open AccessGalleria mellonella as an infection model for Campylobacter jejuni virulence
Author(s) -
Nicola J. Senior,
Mary C. Bagnall,
Olivia L. Champion,
Stuart E. Reynolds,
Roberto M. La Ragione,
Martin J. Woodward,
Francisco J. Salguero,
Richard W. Titball
Publication year - 2011
Publication title -
journal of medical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.91
H-Index - 117
eISSN - 1473-5644
pISSN - 0022-2615
DOI - 10.1099/jmm.0.026658-0
Subject(s) - galleria mellonella , campylobacter jejuni , virulence , microbiology and biotechnology , biology , bacteria , biochemistry , genetics , gene
Larvae of Galleria mellonella (Greater Wax Moth) have been shown to be susceptible to Campylobacter jejuni infection and our study characterizes this infection model. Following infection with C. jejuni human isolates, bacteria were visible in the haemocoel and gut of challenged larvae, and there was extensive damage to the gut. Bacteria were found in the extracellular and cell-associated fraction in the haemocoel, and it was shown that C. jejuni can survive in insect cells. Finally, we have used the model to screen a further 67 C. jejuni isolates belonging to different MLST types. Isolates belonging to ST257 were the most virulent in the Galleria model, whereas those belonging to ST21 were the least virulent.
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