Open AccessDirect 16S rRNA gene sequencing of polymicrobial culture-negative sampleswith analysis of mixed chromatograms
Author(s) -
Gitte Nyvang Hartmeyer,
Ulrik Stenz Justesen
Publication year - 2009
Publication title -
journal of medical microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.91
H-Index - 117
eISSN - 1473-5644
pISSN - 0022-2615
DOI - 10.1099/jmm.0.017269-0
Subject(s) - microbiology and biotechnology , fusobacterium necrophorum , neisseria meningitidis , 16s ribosomal rna , neisseria , streptococcus mitis , biology , prevotella intermedia , prevotella , streptococcus , gene , bacteria , genetics , porphyromonas gingivalis
Two cases involving polymicrobial culture-negative samples were investigated by 16S rRNA gene sequencing, with analysis of mixed chromatograms. Fusobacterium necrophorum, Prevotella intermedia and Streptococcus constellatus were identified from pleural fluid in a patient with Lemierre's syndrome and Neisseria meningitidis and Escherichia coli were identified from a petechia in a patient with meningococcal disease.
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