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Purpose. This study explored the prevalence and characteristics of Enterococcus faecalis biofilm formation by urinary tract infection (UTI) isolates in order to identify virulence factors associated with biofilm formation. Methodology. A total of 113 E. faecalis isolates were collected from UTI patients in Shenzhen, China. The isolates were subjected to multilocus sequence typing based on housekeeping genes. Biofilms were detected by crystal violet staining and the expression levels of the E. faecalis genes were detected by quantitative real-time PCR. Results/Key findings. The main sequence types (STs) were ST16 and ST179 with the ST16 isolates more likely to form strong biofilms than the ST179 isolates ( P=0.008 ). Strong biofilm formation was more frequently detected in aggregation substance ( agg )-positive (+) isolates than in negative (-) isolates ( P=0.033 ). Biofilm formation was also more common in isolates containing enterococcal surface protein ( esp ), or cytolysin A ( cylA )-positive (+) isolates than in isolates negative (-) for these virulence factors. Multivariate regression analysis indicated that cylA [odds ratio (OR), 7.143, P = 0.012 ] was associated with weak biofilm formation, and that agg (OR, 4.471, P=0.004 ) was associated with strong biofilm formation. The expression of cylA was increased (8.75- to 23.05-fold) in weak biofilm, and the expression of agg was greatly elevated (11.99- to 439.10-fold) in strong biofilm isolates when compared to biofilm-negative isolates. Conclusion. ST16 classification was positively associated with strong biofilm formation in E. faecalis as was agg , while cylA was associated with weak biofilm formation.

Characterization of biofilm formation by Enterococcus faecalis isolates derived from urinary tract infections in China