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UnlikeEscherichia coli    ,Campylobacter jejuniis unable to import a range of sugars, including arabinose, which makes common expression vectors, such as pBAD33, non-functional in these bacteria.    Aim   The aim of this study was to investigate whether theE. colitransporters AraE and modified LacY (LacYA177C) would enableC. jejunito uptake arabinose.    Methodology and Results   The respective genes ofE. coliwere constitutively expressed inC. jejunistrain 11168H after integration into the chromosome via homologous recombination. Vectors carrying these genes also contained a reporter gene,  gfp , under the control of the arabinose-inducible promoter, pBAD. These constructs were verified inE. coliby demonstrating the induction of  gfp  in the presence of arabinose. Integration of the genes into one of the rRNA gene clusters was verified by PCR and genome sequencing. The latter also confirmed that the inserted gene clusters contained no mutations. Expression of the  gfp  gene in the presence of arabinose inducer was monitored using fluorescence microscopy of colonies and fluorimetry using both whole cells and lysates.    Conclusion   The results demonstrated the inability ofC. jejunito use arabinose transporters, which are fully functional inE. coli    , suggesting a remarkable difference in the physiology of these bacteria.

Expression of Escherichia coli araE and modified lacY genes in Campylobacter jejuni is not sufficient for arabinose transport