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The temperate bacteriophage AAU2 of ‘Arthrobacter aureus’ C70, isolated from a soil sample, has been purified and characterized. The phage belongs morphologically to the Siphoviridae family. Its genome consists of a linear double-stranded DNA of 45.4 kb with cohesive ends. The attP site and the phage-encoded functions essential for integration were cloned in Escherichia coli as a 5.25 kb BglII-EcoRV fragment. The resulting plasmid vector, non-replicating in ‘A. aureus’, is able to integrate at high frequency into the chromosomes of ‘A. aureus’ and the industrially used coryneform bacteria Brevibacterium lactofermentum and Corynebacterium glutamicum.

aU2, a temperate bacteriophage specific for 'Arthrobacter aureus', whose integrative functions work in other corynebacteria