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The reactions givenby cultures of Aeromonas in the bio- chemical tests commonly used in the diagnostic laboratory often resemble those of Enterobacteriaceae and the differ- entiation of Aeromonas may be difficult. Frequently, how- ever, strains of Aeromonas can be identified accurately if a number of media are employed and if the reactions obtain- ed are compared with those given by members of the various groups of Enterobacteriaceae. Also, flagellar stains are of assistance in this differentiation. In a search for a more rapid, as well as accurate, meth- od of distinguishing Aeromonas cultures, we tried the cyto- chrome oxidase test employed by Gaby and Hadley (1957) for the identification of Pseudomonas aeruginosa. The test media employed by these investigators were nutrient broth tubed in 4.5 ml amounts and nutrient agar or blood agar plates. Cultures to be tested were incubated for 18 to 24 hours at 37°C or at room temperature (24 to 28'C), depend- ing upon which was optimal. Following incubation, 0.2 ml of a 1.0 per cent solution of alpha-naphthol in 95 per cent ethyl alcohol and 0.3 ml of a 1.0 per cent aqueous solution of para-aminodimethylaniline oxalate were added to each broth culture. The cultures were then shaken. The appear- ance of a blue color was indicative of the presence of cyto- chrome oxidase. Plate cultures were tested by allowing a few drops of an equal-parts mixture of the two reagents to flow over isolated colonies. The authors first employed the method as described above but subsequent tests indicated that nutient broth tubed in 1.0 ml amounts gave results comparable to those obtained with larger volumes and long nutrient agar slants yielded superior results. Hence, nutrient agar slant cultures, in- cubated at 37"C, or at a lower temperature if required, for 18 to 20 hours were employed in the present studies. After

The differentiation of Aeromonas and C27 cultures from Enterobacteriaceae