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Pigmentation mutants of Pseudomonas phenazinium unable to synthesize iodinin, or producing it only in reduced amounts, were isolated. The abilities of the mutants to synthesize nine other phenazines were also altered. Cross-feeding experiments and the altered patterns of pigment production suggested metabolic relationships between the phenazine pigments, and a scheme for their biosynthesis is proposed.

Isolation of Pigmentation Mutants of Pseudomonas phenazinium