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METHODS Organism and cultural conditions. Kluyveromyces fragilis (NCYCIOO) was grown in 2 % Oxoid malt extract broth at 15, 30 and 40 "C, with shaking at 160 rev./min. Cultures were grown to the exponential growth phase at each temperature. Under these conditions, the yeasts had a doubling time of 160 min at 15 "C, go min at 30 "C and 120 min at 40 "C. Cell number determinations were carried out using a Thoma haemocytometer. (Duffus & Penman, 1973). Cytochemical and biochemical methods. The time of nuclear division was determined in cells grown at each temperature following Giemsa staining, as described by Duffus & Penman (1973). DNA was estimated in cultures grown at each temperature following the method of Bostock (1970). RNA was estimated using the orcinol method (Mejbaum, 1939).

The Effects of Various Growth Temperatures on Nuclear Division, DNA and RNA in the Budding Yeast, Kluyveromyces fragilis