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SUMMARY: The addition of polymyxin E to washed cell suspensions of a strain of Pseudomonas aeruginosa caused a release of materials which had absorption maxima at 260 mμ., pentose and phosphate from the cells. This release did not occur at 2° and in this respect differed from the leakage resulting when cells were suspended in distilled water or were treated with cetyltrimethylammonium bromide (CTAB). There was an optimum concentration of polymyxin for maximum release of cell constituents, higher concentrations inhibiting the release. A similar release of cell constituents, which was prevented at low temperatures, occurred when cells were suspended in buffer at pH 5·3. Morphological changes resulting from polymyxin treatment have been studied using the electron microscope.

The Release of Soluble Constituents from Washed Cells of Pseudomonas aeruginosa by the action of Polymyxin