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SUMMARY Washed suspensions of three Epidinium spp., grown in vitro and incubated anaerobically in salts solution, released into the medium protein containing hexokinase and an enzyme which lysed Bacillus megaterium, B. subtilis and Micrococcus lysodeikticus. Material from the lysate, containing bound amino acids, was incorporated by Epidinium spp. at a rate that would allow only very slow growth if it were the sole source of nitrogen. Although there was no massive cell lysis the enzymes were liberated as the result of a release of whole cell contents by an unknown mechanism. In the presence of other rumen protozoa, protein, but no lytic enzyme, appeared in the medium. No good evidence was obtained for the existence in the rumen of a lytic enzyme associated with the presence of Epidinium SPP.

The Utilization of Bacillus megaterium and the Release of a Lytic Enzyme by Three Epidinium spp. Isolated from the Rumen