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Conidia which are not 'wettable' in water, such as those of Aspergillus nidulans, stick together in clusters of irregular shapes in liquid suspension due to the presence of lipids on their outer walls. In order to prepare a homogeneous single-celled suspension, a 'wetting agent ' such as Tween-80 is used. Such agents can break down the lipid barrier to diffusion of water-soluble compounds and so allow self-inhibitors of spore germination to diffuse out of the cell, as has been demonstrated in Puccinia graminis (Allen, 1955) and Aspergillus nidulans (Scott, Alderson & Papworth, 1972). In the latter organism progressive loss of the germination inhibitor from the conidium results in a corresponding increase in sensitivity to radiation; constant and maximal lethality is exhibited only after the inhibitor has been effectively removed (Scott et al. 1972). Since the Tween-80 cannot be removed before conducting assays for radiation effects, an alternative method of preparing a homogeneous single-celled suspension of inhibitor-depleted spores was sought. The germination inhibitor of Puccinia graminis has been identified as the cis and trans isomers of methyl ferulate (Macko, Staples, Allen & Renwick, 1972). This inhibitor was extracted from an aqueous suspension of the spores by diethyl ether, a solvent used by other workers to obtain lipid-free spores of Aspergillus and Penicillium for physiological studies (Koval, Sivers & Redchits, 1968). Diethyl ether was therefore tested as a possible alternative agent to Tween-80 for studies of radiation response.

A Rapid Method for Preparing Single-cell Suspension of Aspergillus Conidia Depleted in Germination Inhibitor