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SUMMARY: The sensitivity of Saccharomyces cerevisiae to sphaeroplast formation has been examined during the transition from the exponential phase of growth to the stationary phase. Exponential-phase yeasts are sensitive to sphaeroplast formation while stationary yeasts are resistant. During the transition period, there is a rapid increase in resistance to sphaeroplast formation. This increase can be inhibited by treatment with either cycloheximide or 5-fluorouracil. It is suggested that the resistance to sphaeroplast formation characteristic of stationary-phase yeasts is the result of a specific modification of the yeasts during the transition period which is dependent on both RNA and protein synthesis.

Sphaeroplast Formation in Yeast During the Transition from Exponential Phase to Stationary Phase