English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Plus monthly

Global: Zendy Tools annual

Global: Zendy Tools monthly

Global: Zendy Free Plan

SUMMARY: An aseptic technique is described for obtaining small hanging drops on the surface of coverslips, using only apparatus that is cheap and easy to obtain. The technique has been used to demonstrate the growth of Nocardia salmonicolor on liquid paraffin droplets. Of all the methods used for studying the growth and development of micro- organisms in liquid culture in undisturbed condition the hanging drop is probably the best. The ordinary hanging-drop method, as used for routine examination of gross bacterial morphology and motility, yields unavoidably large drops and is unsuited to the study of the growth and development of a few cells. Microbiologists requiring more stringent control of drop size have turned to the technique of micromanipulation, but for the average worker the apparatus required is too time-consuming and tedious to construct. The technique described below is offered for those workers who do not require the refinements of micromanipulation methods. The apparatus required is cheap and easy to obtain. METHOD The method consists of spraying a suspension of organisms, in the medium in which they are to be studied, on to the surface of coverslips. The suspension is made of such a density that the droplets formed on the coverslip surface contain a few cells each. The coverslip is then inverted over a well slide containing liquid paraffin and incubated at the required temperature.

A Simple Method for Producing Microcultures in Hanging Drops with special reference to Organisms Utilizing Oils