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SUMMARY: Ninety-three coryneform bacteria were tested with various acids and bases and on the basis of these tests, five of the strains were identified as Brevibacterium linens. This identification correlated with that made by other methods, thus confirming the suggestion of Grecz & Dack (1961) that B. linens could be identified by certain colour reactions. Further studies indicated that these reactions are probably associated with the presence of a specific carotenoid-like substance located in the bacterial membrane.

Taxonomically Significant Colour Changes in Brevibacterium linens Probably Associated with a Carotenoid-like Pigment