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SUMMARY: The production of staphylococcal α-toxin in a synthetic amino acid medium was stimulated by a factor from yeast autolysate which was identified as histidine by chromatographic methods. Addition of histidine to the medium gave an early appearance of α-toxin, while serine and glycine stimulated its production in the late growth phase. The stimulatory capacity of serine and glycine in relation to their function as precursors in the synthesis of histidine is discussed.

Production of Staphylococcal -Toxin in a Defined Medium and Identification of a Stimulating Factor from Yeast Extract