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Bacillus amyloliquefaciens readily forms large aggregates (Welker & Campbell, I 967) and dry weights determined indirectly by bacterial counts, turbidimetry or nephelometry (Mallette, 1969) are not satisfactory and consequently the direct determination of dry weight has always been used (Coleman & Elliott, 1962). We have now developed a rapid turbi- dimetric technique for this determination which gives comparable results to those obtained by the direct method. METHOD Samples of bacterial culture, usually in the range 2 to 5 m1, were centrifuged for 2 min at 2500 g and resuspended to the original volume in distilled water. The resulting suspension was then treated with an ultrasonic disintegrator (100 W, 20 kHz; M.S.E. Ltd, Crawley, Sussex) for I min. A sample, usually I ml, of the disrupted preparation was then diluted with distilled water to 2 ml and z ml of 10 % (w/v) trichloroacetic acid was added. After thorough mixing the extinction of the resulting turbid suspension was measured at 600 nm in a cuvette of I cm light path by means of a Unicam SP 500 spectrophotometer.

A Rapid and Convenient Method for Determining the Dry Weight Content of Bacteria Which Show a Tendency to Form Large Aggregates