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SUMMARY The Escherichia coli K, B and phage P I host specificity genes (hsp) were introduced into Salmonella typhimurium by means of E. coli Hfr x S. typhimurium F- crosses. The three systems were found to govern modification and restriction of phages P22 and L. The restriction coefficients were comparable to that of h for the PI system and were lower (about 100) for the K and B systems. Phage grown on hspK+ and hspB+ recombinants was restricted by S. typhimurium independently of the re- striction governed by the LT system. This observation suggested that S. typhimurium possesses a previously undetected hsp locus allelic to those of E. coli K and B.

Expression of the Escherichia coli K, B and Phage P1 DNA Host Specificities in Salmonella typhimurium