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SUMMARY: Lysine auxotrophs have been isolated from Pseudomonas aeruginosa 8602. Three of the mutants were deficient in diaminopimelate decarboxylase and accumulated meso- and ll-diaminopimelic acid (DAP) but otherwise were indistinguishable from the parent strain. The fourth mutant required lysine for optimal growth, grew slowly on meso- but not ll-DAP, and the DAP which accumulated in large amounts was solely the ll-isomer. This mutant was deficient in diaminopimelate epimerase. No significant differences were detected between its wall composition and that of the parent strain but it was particularly sensitive to carbenicillin.

Diaminopimelic Acid and Lysine Auxotrophs of Pseudomonas aeruginosa 8602