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SUMMARY DNA breakdown was detected 3 to 4 min. after addition of colicin E2 to sensitive cells; inhibition of cell division followed 5 to 10 min. later, but inhibition of DNA synthesis was observed only after several more minutes. Adsorption of E2, which takes place even at 4", led to the formation of a specific surface complex (I). Complex I did not promote DNA breakdown. We suggest that the transition from this complex to a surface complex (11) which promoted DNA breakdown depended upon several factors which include temperature, concentration of E 2, specific membrane proteins and, under certain conditions, high concentrations of extracellular KH2P04. The formation of complex I1 did not depend on concomitant DNA or protein synthesis. The continued promotion of DNA breakdown by complex I1 and its associated nuclease was blocked by inhibition of energy metabolism. In addition, the removal of E2 from the cell surface by trypsin treatment during the early stages of the process greatly decreased the rate of DNA breakdown. Ez-induced DNA breakdown, which appears to commence from a limited number of chromosomal sites, proceeded normally in UVr-, RecB-, RecC-, Hsr-, HSS-, PolA- and in several tsDNA replication mutants.

journal of general microbiology/journal of general microbiology

Induction of DNA Breakdown and Inhibition of Cell Division by Colicin E2. Nature of Some Early Steps in the Process and Properties of the E2-specific Nuclease System