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SUMMARY Saprophytic cultures obtained from uredospores of Puccinia graminis f. sp. tritici race I 26-~~~-6,7 varied. Cultures were placed in two general categories : those which formed macroscopic colonies within 2 weeks of inoculation, and those which formed colonies 4 or more weeks after inoculation. The fast- appearing colonies contained binucleate cells and were the more common; growth ceased by staling or formation of spore-bearing stromata. Slow- appearing colonies were formed at an erratic and low incidence; they were uninucleate, showed much less tendency to stale or form stromata than did fast-appearing colonies, and could be propagated by serial subcultures. Varia- tion of uninucleate colonies during maintenance in axenic culture is described.

Variant Forms of Saprophytic Mycelium Grown from Uredospores of Puccinia graminis f. sp. tritici