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SUMMARY In the hypha of Paracoccidioides brasiliensis the succinic dehydrogenase activity is localized in the mesosomes, plasma membrane and mitochondria. Short permanganate fixation after incubation enhances the positive cyto- chemical reaction. The higher activity is localized in mesosomes and it is observed as high electron-dense zones and concentric ring-like patterns. The membrane shows a spotty reaction and the mitochondrion shows a positive zone in the intracristae space. The mycelial phase of Paracoccidioides brasiliensis (IVIC-~b9) was inoculated into a GGY medium (glycine I %, glucose 2 %, yeast extract 0.2 %) in Erlenmeyer flasks which were placed on a reciprocal shaker at JOO oscillationslmin., stroke amplitude 5 cm., at 20 to 22". Four-day-old samples were harvested by low speed centrifugation and incubated for 30 to 50 min. in the following mixture: 3-5 ml. 0.8 M-sodium succinate, tetranitro blue tetrazolium (TNBT) I mg./ml., which had been previously dissolved in N,N- dirnethylformamide (Sedar, Rosa & Tsou, 1962; Sedar & Burde, 1965). For controls, sodium malonate (0.5 g.) was added to the incubated mixture as a competitive inhibi- tor; the material was incubated without substrate; and the enzyme was inactivated

Demonstration of Succinic Dehydrogenase in the Mesosomes of the Mycelial Phase of Paracoccidioides brasiliensis