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SUMMARY Three methods of detecting respiratory variation in Neurospora crassa have been investigated. They involved the addition of dyes (pontamine sky blue and eosin yellowish) tellurite or tetrazolium to a complete medium con- taining ethionine. The latter prevented conidiation and enabled the colours of the colonies to be seen clearly. These methods were used to distinguish nuclear and cytoplasmic cytochrome mutants (cyt-I, cyt-2, mi-I, mi-3, SG-3 and SG) from wild-type colonies on the basis of colour. Tetrazolium violet (2.5-diphenyl-3-(1-napthyl)-tetrazolium chloride) incorporated into modi- fied Nagai medium with ethionine is recommended for distinguishing cyto- plasmic mutants of Neurospora from wild-type. Certain other nuclear mutants were also distinguishable from wild-type in some of these tests. These were ac-I, ac-2, acr-3, ad-4, leu-5, nit-I, OXD-I. Thirty-two wild-type strains in reciprocal crosses were tested on these media to find whether these tests would distinguish any nuclear or cytoplasmic variation. No new cyto- plasmic variants were found. Three new nuclear genes, Bm (mauve on dye media), Tet-R (ability to reduce tetrazolium) and su- (suppressor of mi-3) are described and have been mapped. All three loci were linked to the mating type locus on chromosome I. Tet-R was found in only certain wild-type strains of mating type a (e.g. 74-0~8-1a), and Tet-W (unable to reduce tetrazolium) was found in all wild-type strains of mating type A (e.g. 74-0~23-1A) and certain wild-types of mating type a;f, a suppressor of mi-I, also suppressed SG-3 and SG, but not mi-3; su- suppressed mi-3, but none of the other cytoplasmic mutants.

Methods for the Study of Nuclear and Cytoplasmic Variation in Respiratory Activity of Neurospora crassa, and the Discovery of Three New Genes