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SUMMARY: Various strains originally designated as members of the genera Micrococcus, Sarcina, and Staphylococcus were able to transform adenine, histidine, and tryptophan auxotrophs of Micrococcus lysodeikticus to prototrophy at relatively high frequencies. These included certain strains of M. flavocyaneus, M. flavus, M. luteus, M. lysodeikticus, M. sodonensis, Sarcina flava, S. lutea, S. subflava, Staphylococcus afermentans and St. flavocyaneus. Many of the above strains were adenine auxotrophs and produced low frequencies of prototrophic recombinants when crossed with the adenine-dependent M. lysodeikticus strain isu. Prototrophic strains of M. conglomeratus, M. radiodurans, M. roseus, M. rubens, M. varians, St. aureus and St. epidermidis failed to transform M. lysodeikticus in these studies. Prototrophic pigment mutants of M. lysodeikticus and S. lutea were able to doubly transform yellow auxotrophs of M. lysodeikticus to prototrophy and donor colour.

Transformation of Micrococcus lysodeikticus by Various Members of the Family Micrococcaceae