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SUMMARY: Among 20 locally isolated fungi, Aspergillus niger isolate no. 58 proved to be suitable for the production of active extracellular milk-clotting enzyme. The addition of acetate buffer to the reaction mixture enhanced the clotting of milk by the enzyme whereas citrate + phosphate buffer hindered the process. The general properties of the crude enzyme were studied. Precipitation with ammonium sulphate, ethanol, acetone and tannin showed that ammonium sulphate was unsuitable for precipitation while the other precipitants produced sufficiently active fractions.

Production of a Milk-clotting Enzyme Preparation by Aspergillus niger and the Effect of Various Factors on its Activity