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SUMMARY: Ethidium bromide, a trypanocidal drug affecting nucleic acid synthesis, was found to be a powerful agent in eliminating some antibiotic resistance in bacteria. In staphylococci, penicillinase production was eliminated in mercury-resistant organisms, but not in mercury-sensitive ones. Among enterobacteria, two resistance factors showing the same resistance pattern were differently eliminated, and correlation between elimination and transfer of resistance factors was not always observed. F’-lac+ factor was also eliminated by ethidium bromide in Escherichia coli KI2. Elimination of antibiotic resistance was observed generally at high frequency, and could be better reproduced than with acridine dyes.

Elimination by Ethidium Bromide of Antibiotic Resistance in Enterobacteria and Staphylococci