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SUMMARY: Clostridium tetanomorphum grew in medium containing yeast extract when supplied with glutamate, histidine, glucose, maltose or pyruvate. Measurements of QH2 values and specific activities for two enzymes, phosphofructokinase and β-methylaspartase indicated that the glucose and glutamate fermentations were inducible. With glucose, the onset of active glucose metabolism was delayed in preference for energy sources provided by yeast extract. The inhibitory mechanism which was responsible for this delay did not appear to be a typical enzyme repression but rather a catabolite inhibition. No significant changes in the cobalamin content of the organisms accompanied growth with different substrates.

Deferred Metabolism of Glucose by Clostridium tetanomorphum