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SUMMARY: Under defined experimental conditions plasmodia of Didymium nigripes began to differentiate into fruiting bodies 16-18 hr after transfer to bacteria-free agar. The process has been divided into a number of easily recognizable stages and the duration of each stage determined. Plasmodia lost ability to continue vegetative growth at a time before the actual fruiting bodies began to form.

Observations on the Differentiation of Plasmodia into Fruiting Bodies by the True Slime Mould, Didymium nigripes