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SUMMARY Water-insoluble complexes of ganglioside with cerebroside fixed tetanus toxin at low concentrations (a few LD 5011-111.) of toxin. A complex containing 25% ganglioside with cerebroside was 50 times better at fixing toxin than complexes containing either 2 % or 50 % ganglioside. A complex containing 25 yo of a mixture of the gangliosides GI,, and G,, was 12 times better at fixing toxin than a similar complex with gangliosides GI and GII. Complexes of ganglioside with sphingomyelin and lecithin fixed toxin to a slight extent, while complexes with tripalmitin and cholesterol did not fix toxin. The complex of cerebroside and ganglioside, containing 25 % ganglioside, did not fix strychnine, serotonin, botulinum toxin or plasma albumin.

The Effect of Cerebroside and Other Lipids on the Fixation of Tetanus Toxin by Gangliosides