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SUMMARY Pseudomonas aeruginosa 8602 was grown in continuous culture under steady-state conditions in a carbon-limited medium containing either 20 nm- acetamide or 20 mM-acetamide + I o mM-succinate. The amidase specific acti- vity was measured at various dilution rates and found to have a sharp peak at a dilution rate of 0.30-0-35 hr-l. Fully constitutive mutants (C 11 and L9) gave curves for amidase activity with the highest values at very low dilution rates (0~05-0~10 hr-l) and these decreased as the dilution rate in- creased. A semi-constitutive mutant (C 17) gave a curve intermediate between that of the wild-type strain and the fully constitutive mutant (C I I). Mutants with decreased sensitivity to catabolite repression by succinate gave curves which declined less steeply at the higher dilution rates. Mutant L 9, a fully constitutive mutant with decreased sensitivity to catabolite repression, had higher specific activities than mutant C 11 at the equivalent dilution rates. Mutant L I I, an inducible mutant with decreased catabolite repressibility, had higher amidase specific activities at high dilution rates than the wild- type inducible strain. It is concluded that in continuous culture under steady-state conditions the specific activity of the inducible amidase of P. aeru- ginosa is determined by the balance between induction and catabolite repres- sion and that catabolite repression is directly related to the growth rate of the culture.

Catabolite Repression and the Induction of Amidase Synthesis by Pseudomonas aeruginosa 8602 in Continuous Culture