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SUMMARY: Crude tetanus toxin paralyses the cholinergic innervation of the sphincter pupillae of the rabbit eye. To test whether this activity was due to tetanospasmin or to a non-spasmogenic fraction with a peripheral effect, the action of crude tetanus toxin and of the non-spasmogenic fraction prepared from it were compared in rabbits. The paralytic action of the crude toxin was not due to its content of the non-spasmogenic fraction and was probably due to tetanospasmin itself. A convenient method for the preparation of a mixture of the four most abundant gangliosides is given. The preparation of a ganglioside cerebroside complex and its use for the removal of tetanospasmin from a solution of crude tetanus toxin are described.

The Effect of the Treatment of Crude Tetanus Toxin with Ganglioside Cerebroside Complex on Sphincter Paralysis in the Rabbit's Eye