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SUMMARY: Preliminary agglutination and precipitin tests to which 60 newly isolated strains of Pseudomonas aeruginosa were submitted confirmed the existence of at least five serological groups. Ouchterlony precipitin tests and immuno-electrophoresis experiments, made with the concentrated trichloroacetic acid extracts of eight strains representative of these groups, revealed the antigenic heterogeneity of this species while the presence of at least 12 antigens was demonstrated. One of these was common to seven of the eight strains. Each of five strains, representative of five different serological groups, possessed at least one antigen peculiar to itself. The remaining ones appeared to be distributed at random among the strains. At least three different antigens migrated towards the positive electrode, of which two appeared to be electrophoretically inhomogeneous.

The Antigens of Pseudomonas aeruginosa Studied by the Ouchterlony Technique and Immuno-electrophoresis