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SUMMARY A plaque assay method is described for the titration of the infective ribonucleic acid (RNA) of foot-and-mouth disease (FMD) virus. The method depends on the dilution of the RNA in salt solutions of high molarity and the treatment of the BHK 21 cell monolayers used with M-NaCl. The method is reproducible and is at least as sensitive as other methods of titration. The extraction of RNA in the presence of EDTA gave titres corresponding to 0*1% of the titre of the intact virus. RNA prepared from virus suspensions containing EDTA retained about 50 yo of initial infectivity for 7 days at 4' but for longer periods it was best preserved in 70 % (v/v) ethanol at - 20'.

The Assay, Extraction and Storage of Infective Ribonucleic Acid from Foot and Mouth Disease Virus