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SUMMARY Strains of Streptococcw bovis, Selenomonas ruminantiurn, and an an- aerobic lipolytic bacterium (5 s) have been grown under carbohydrate- limiting conditions in continuous culture for long periods. With s. rumi- nuntium and bacterium 5 s the fermentation products varied with growth rate. Yield of organism in continuous culture of all three bacteria showed a maximum at a particular growth rate. The yield of S. rumimafitium was much higher in continuous culture at optimum growth rate than in batch culture, and was also higher than can be explained on present concepts of the energy from fermentation available for growth. The various results are discussed in the light of results obtained with other bacteria and the con- ditions obtaining in the rumen.

Continuous Culture of Some Anaerobic and Facultatively Anaerobic Rumen Bacteria