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SUMMARY: Several methods for growing animal cells in suspension culture were examined, to find the most efficient in terms of cells produced in a given time for the minimum of medium and attention. Continuous medium flow was more efficient than batch-culture, and the preferred system was to add medium to a culture vessel regularly in small doses via a time-switch-controlled solenoid closure at a rate similar to the growth rate; a turbidimetric safety device ensured that cell density did not drop below levels permitting growth. A mixture of galactose or fructose (6 g./1.) and glucose (2 g./1.) allowed better pH control than glucose alone (6 g./1.). Doubling times of 14r–16 hr. were obtained on occasion; the gas-phase oxygen concentration for fastest growth depended on cell density and was frequently less than atmospheric, suggesting that these cells can behave as microaerophils.702

The Bulk Growth of Animal Cells in Continuous Suspension Culture