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SUMMARY: Optimal conditions for the adsorption of bacteriophage λ22 by Escherichia coli strain K112 require 0·02M-MgSO4 and a pH value of the order 6·5. With a multiplicity of input of 20 phage particles per bacterium, 99% of the bacteria are infected and at least 90% are lysogenized. Irradiation of the cells with ultraviolet light immediately prior to infection causes them to give the lytic rather than the lysogenic response.

Conditions for the Infection of Escherichia coli with Lambda Phage and for the Establishment of Lysogeny