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SUMMARY: Mycobacteria were grown in rocked ⊥ tubes (Monod) in a medium which contained asparagine, glycerol, 1·0% (w/v) Tween 80 and no albumin fraction V. This permitted abundant well-dispersed diffuse growth. The amount of growth could be measured photometrically.

Cultivation of Mycobacteria Rocked in Non-Protein Medium Containing a High Concentration of Tween 80