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Five rumen strains of Streptococcus bovis all required biotin for good anaerobic production of dextran from sucrose in a simple acetate + bicarbonate + amino acids medium. Three of these strains required at least pantothenate also. When phosphate replaced acetate as a buffer, dextran production did not occur unless a soluble protein was also supplied in the medium.

The Nutritional Requirements of Rumen Strains of Streptococcus bovis Considered in Relation to Dextran Synthesis from Sucrose