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SUMMARY: A comparison of an indirect method of counting virus particles (i.e. the ‘absolute assay’ haemagglutination method) with direct electron-microscope counts showed that the indirect method underestimated the total virus particle count by a factor of about 10. Details of technique and possible sources of error in making counts are discussed.

The Assay of Influenza Virus Particles by Haemagglutination and Electron Microscopy