English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

In the unicellular cyanobacterium Synechococcus sp. PCC 7942, ammonium/methylammonium transport activity has been characterized but ammonium transport genes have not been described. The amt1 gene encoding a permease responsible for high-affinity [14C]methylammonium transport in Synechococcus sp. PCC 7942 was cloned and inactivated. The Amt1 permease appeared essential to take up ammonium when it was present at low concentrations in the external medium and might also be involved in recapture of ammonium leaked out from the cells. Expression of amt1, which was induced in the absence of ammonium and also influenced by the inorganic carbon supply, was dependent on the NtcA transcriptional regulator. The promoter of amt1 was found to exhibit the structure of NtcA-activated promoters, and specific binding of purified NtcA to amt1 promoter sequences was observed. The results of this study indicate that amt1 belongs to the NtcA regulon and that NtcA may respond to both nitrogen and carbon availability.

microbiology

The NtcA-activated amt1 gene encodes a permease required for uptake of low concentrations of ammonium in the cyanobacterium Synechococcus sp. PCC 7942 The GenBank accession number for the nucleotide sequence of the amt1 gene described in this paper is AJ311900.